Counting Constituents in Molecular Complexes by Fluorescence Photon Antibunching
نویسندگان
چکیده
منابع مشابه
Imaging of Single Photon Emitter Fluorescence and Photon Antibunching
In this experiment we demonstrate the Confocal Microscope fluorescence imaging of various single photon emitters like carbon nanotube, quantum dots and color centre in nano-diamonds. We also measure their spectrum to characterize them. Finally we use the Hanbury Brown and Twiss Setup to test if the photons emitted from our source has antibunching properties.
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Fluorescence antibunching is a well-known technique for determining the number of independent emitters per molecule or molecular complex. It was rarely applied to autofluorescent proteins due to the necessity of collecting large numbers of fluorescence photons from a single molecule, which is usually impossible to achieve with rather photolabile autofluorescent proteins. Here, we measure fluore...
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We present a time-correlated single photon counting (TCPSC) technique that allows time-resolved multi-wavelength imaging in conjunction with a laser scanning microscope and a pulsed excitation source. The technique is based on a four-dimensional histogramming process that records the photon density over the time of the fluorescence decay, the x-y coordinates of the scanning area, and the wavele...
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We observed photon antibunching in the fluorescent light emitted from a single nitrogen-vacancy center in diamond at room temperature. The possibility of generating triggerable single photons with such a solid-state system is discussed.
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The nature of the highly efficient energy transfer in photosynthetic light-harvesting complexes is a subject of intense research. Unfortunately, the low fluorescence efficiency and limited photostability hampers the study of individual light-harvesting complexes at ambient conditions. Here we demonstrate an over 500-fold fluorescence enhancement of light-harvesting complex 2 (LH2) at the single...
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ژورنال
عنوان ژورنال: IEEE Journal of Selected Topics in Quantum Electronics
سال: 2007
ISSN: 1077-260X
DOI: 10.1109/jstqe.2007.903193